Fiche publication
Date publication
février 2025
Journal
Nanoscale
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DECREAU Richard
Tous les auteurs :
Saou S, Moreau M, Lioret V, Berrou A, Hernandez-Garcia M, Decréau RA
Lien Pubmed
Résumé
Liposomes used for the study were prepared and carefully characterized multiple times until all batches indicated the same characterization data (DOPC/cholesterol derivative (1 : 1.15 mol%), 14 mg(DOPC) mL, = 130 nm, 2 × 10 liposome per nm of prepared batch, polydispersity index PDI = 0.1). The study shows that such a liposome suspension raises the yield in Cherenkov Radiation (CR) by 1.6-fold when in presence of [Ga]-GaCl, an efficient CR emitter (beta particle energy = 1800 KeV). Also, liposomes were found to prevent aggregation of a water-soluble phthalocyanine-pyranine PcPy dyad upon encapsulation, leading to its spectacular fluorescence restoration. Altogether, upon efficient Ga-radiolabelling of NODAGA-chelate immobilized at the liposome surface (99% radiolabelling yield, radio-TLC showed) encapsulating PcPy dyad ( = 97 μM, = 300 μM), subsequent Cherenkov Radiation Energy Transfer (CRET) at the dyad antenna occurred. Internal energy transfers and fluorescence emission from the emitter dyad led to a 2.6-fold raise in radiance measured in the near-infrared (NIR) window ( 400 nm pseudo-Stokes shift). A similar raise in radiance was measured in the green window when encapsulation was achieved with eosin at the same rate. Liposome were found to be stable in PBS over 7 days regardless of the encapsulated fluorophore (no raise in diameter, no release of encapsulated dyes measured after Sephadex and FPLC repurification sequence), with some decay over 22 hours in a PBS/fetal calf serum mixture (1 : 1 vol.).
Référence
Nanoscale. 2025 02 3;:
