Fiche publication
Date publication
février 2025
Journal
The Analyst
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BREZILLON Stéphane
,
Pr RAMONT Laurent
,
Pr SOCKALINGUM Ganesh
,
Dr AUDONNET Sandra
Tous les auteurs :
Pantazi L, Untereiner V, Rosales P, Rivet R, Audonnet S, Proult I, Ramont L, Sockalingum GD, Brézillon S
Lien Pubmed
Résumé
Ovarian cancer is the most lethal cancer among gynaecological malignancies. Due to the lack of early symptoms and screening tools, patients are diagnosed in advanced stages. Cancer invasion and metastasis through the extracellular matrix (ECM) are enhanced by tumour cell Extracellular Vesicles (EV). The aim of this study was to characterise the EVs derived from two ovarian cancer cell lines (ES2 and SKOV3) using biochemical and vibrational spectroscopic approaches. EVs were prepared by ultracentrifugation and characterised by Nanoparticle Tracking Analysis. Their surface proteins were assessed by MACSPlex EV kit for human exosomes. The presence of MMP14 and integrin subunits was evaluated in EVs and cell protein extracts by Western immunoblotting. Both EVs and cells were measured by Fourier transform infrared spectroscopy (FTIR) and data were analysed by hierarchical cluster analysis (HCA). Spectral differences were observed in the lipids and polysaccharides regions both between the SKOV3 and ES2 cells and their corresponding EVs, which allowed a good delineation by HCA. The differences in the biochemical data were confirmed by similar and specific features exhibited in their respective infrared spectral signatures. ES2 EVs exhibited an enrichment in MMP14 in agreement with the aggressiveness of this ovarian cancer metastatic cell line.
Référence
Analyst. 2025 02 28;:
