Fiche publication
Date publication
novembre 2018
Journal
Biosensors
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LESNIEWSKA Eric
,
Dr BOURILLOT Eric
Tous les auteurs :
Ionescu RE, Selon R, Pocholle N, Zhou L, Rumyantseva A, Bourillot E, Lesniewska E
Lien Pubmed
Résumé
Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm² of Hsp70.
Mots clés
ITO functionalization, biosensor, label-free microwave detection, molecular chaperone Hsp70
Référence
Biosensors (Basel). 2018 11 27;8(4):
