Identification of antigenic proteins using an approach and investigation of their polymorphism.

Fiche publication

Date publication

mars 2025

Journal

Microbiology spectrum

Auteurs

Membres identifiés du Cancéropôle Est :
Pr VILLENA Isabelle

Tous les auteurs :
Denis J, Gommenginger C, Beal L, Cimon B, Deleplancque AS, Fricker Hidalgo H, L'Ollivier C, Paris L, Pelloux H, Pomares C, Houze S, Pfaff AW, Villena I, Villard O

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/40135885

Résumé

is a pathogen characterized by a large variety of strains whose virulence and clinical severity are likely linked to their genotype. Currently, the strains are genotyped using restriction fragment length polymorphism, multilocus sequence typing, and microsatellite markers. This typing requires the strain's DNA, which is difficult to obtain. A serotyping test could overcome the constraints of genotyping, the challenge being to identify type-specific proteins. We identified immunogenic proteins from co-immunoprecipitations for three tachyzoite strains (strain FOU from Africa 1 type, ME49 from Type II, and VEG from Type III) with hyperimmune murine sera and conducted an polymorphism search for the identified proteins. A variant calling analysis was conducted on the next-generation sequencing sequences of 117 isolates with the objective of identifying mutations present in the genes encoding the antigenic proteins previously identified. A total of 727 immunogenic proteins were identified, including 16% dense granule protein (GRA), rhoptry protein (ROP/RON), and surface antigen protein (SAG). Genetic analysis revealed the presence of 36 single-nucleotide polymorphisms (SNPs) in over 70% of isolates belonging to the same type, while less than 30% of isolates belonging to the other types exhibited these polymorphisms. Of these, only 15 are located on coding DNA sequence regions, while four are located on genes encoding apicomplexan proteins: two SNPs on the ROP5 gene and two on the ROP7 gene. The results of this study indicate that a significant number of immunogenic proteins can be identified using an approach. The study identified SNPs that could be genotype-specific.