Fiche publication
Date publication
février 2013
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GUEANT Jean-Louis
,
Pr VIGNAUD Jean-Michel
,
Pr GAUCHOTTE Guillaume
,
Pr GERMAIN Adeline
Tous les auteurs :
Bressenot A, Pooya S, Bossenmeyer-Pourie C, Gauchotte G, Germain A, Chevaux JB, Coste F, Vignaud JM, Gueant JL, Peyrin-Biroulet L
Lien Pubmed
Résumé
Dietary methyl donors and their genetic determinants are associated with Crohn's disease risk. We investigated whether a methyl-deficient diet (MDD) may affect development and functions of the small intestine in rat pups from dams subjected to the MDD during gestation and lactation. At 1 month before pregnancy, adult females were fed with either a standard food or a diet without vitamin B12, folate and choline. A global wall hypotrophy was observed in the distal small bowel (MDD animals 0.30 mm v. controls 0.58 mm; P< 0.001) with increased crypt apoptosis (3.37 v. 0.4%; P< 0.001), loss of enterocyte differentiation in the villus and a reduction in intestinal alkaline phosphatase production. Cleaved caspase-3 immunostaining (MDD animals 3.37% v. controls 0.4%, P< 0.001) and the Apostain labelling index showed increased crypt apoptosis (3.5 v. 1.4%; P= 0.018). Decreased proliferation was observed in crypts of the proximal small bowel with a reduced number of minichromosome maintenance 6 (MDD animals 52.83% v. controls 83.17%; P= 0.048) and proliferating cell nuclear antigen-positive cells (46.25 v. 59 %; P= 0.05). This lack of enterocyte differentiation in the distal small bowel was associated with an impaired expression of beta-catenin and a decreased beta-catenin-E-cadherin interaction. The MDD affected the intestinal barrier in the proximal small bowel by decreasing Paneth cell number after immunostaining for lysosyme (MDD animals 8.66% v. controls 21.66%) and by reducing goblet cell number and mucus production after immunostaining for mucin-2 (crypts 8.66 v. 15.33%; villus 7 v. 17%). The MDD has dual effects on the small intestine by producing dramatic effects on enterocyte differentiation and barrier function in rats.
Référence
Br J Nutr. 2013 Feb 28;109(4):667-77
