Towards the identification of two glycoproteins involved in the stomatal deregulation of downy mildew infected grapevine leaves.

Date publication

juin 2015

Auteurs

Membres identifiés du Cancéropôle Est :
Mme TRUNTZER Caroline

Tous les auteurs :
Guillier C, Gamm M, Lucchi G, Truntzer C, Pecqueur D, Ducoroy P, Adrian M, Heloir MC

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/26106900

Résumé

Stomata remain abnormally opened and unresponsive to abscisic acid in grapevine leaves infected by downy mildew. This deregulation occurs from 3 days post inoculation and increases concomitantly with leaf colonization by the pathogen. Using epidermal peels, we demonstrated that the active compound involved in this deregulation is located in the apoplast. Biochemical assays showed that the active compound present in the apoplastic fluids isolated from Plasmopara viticola infected grapevine leaves (IAF) is a CysCys bridge-independent, thermostable and glycosylated protein. Fractionation guided assays based on chromatography / stomatal response and proteomic analysis allowed the identification of both plant and pathogen proteins in the active fraction obtained from IAF. Further in silico analysis and discriminant filtrations based on the comparison between predictions and experimental indications lead to the identification of two V. vinifera proteins as candidates for the observed stomatal deregulation.

Référence

Mol Plant Microbe Interact. 2015 Jun 24.