Fiche publication
Date publication
août 2012
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUJARDIN Denis
,
Dr DULUC Isabelle
,
Dr FREUND Jean-Noël
Tous les auteurs :
Bellis J, Duluc I, Romagnolo B, Perret C, Faux MC, Dujardin D, Formstone C, Lightowler S, Ramsay RG, Freund JN, De Mey JR
Lien Pubmed
Résumé
The stem cells (SCs) at the bottom of intestinal crypts tightly contact niche-supporting cells and fuel the extraordinary tissue renewal of intestinal epithelia. Their fate is regulated stochastically by populational asymmetry, yet whether asymmetrical fate as a mode of SC division is relevant and whether the SC niche contains committed progenitors of the specialized cell types are under debate. We demonstrate spindle alignments and planar cell polarities, which form a novel functional unit that, in SCs, can yield daughter cell anisotropic movement away from niche-supporting cells. We propose that this contributes to SC homeostasis. Importantly, we demonstrate that some SC divisions are asymmetric with respect to cell fate and provide data suggesting that, in some SCs, mNumb displays asymmetric segregation. Some of these processes were altered in apparently normal crypts and microadenomas of mice carrying germline Apc mutations, shedding new light on the first stages of progression toward colorectal cancer.
Référence
J Cell Biol. 2012 Aug 6;198(3):331-41
