Fiche publication
Date publication
juillet 2012
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MORAS Dino
,
Dr POTERSZMAN Arnaud
,
Dr ROCHEL-GUIBERTEAU Natacha
,
Dr RUFF Marc
,
Dr OSZ-PAPAI Judit
Tous les auteurs :
Osz J, Pradeau-Aubreton K, Drillien R, Troffer-Charlier N, Kolb-Cheynel I, Poterszman A, Ruff M, Moras D, Rochel N
Lien Pubmed
Résumé
Purified protein expression level and quality are contingent upon specific host expression systems. This differential production is particularly observed for proteins of high molecular weight, hampering further structural studies. We developed an expression method aimed at producing proteins in Escherichia coli, insect, and mammalian systems. Our novel protocol was used to produce in large scale the full-length 160-kDa steroid receptor coactivator 1 (SRC-1), a coregulator of nuclear receptors. The results indicate that we can produce biologically active human SRC-1 in mammalian and insect cells in large scale.
Référence
Anal Biochem. 2012 Jul 15;426(2):106-8
