Capillary electrophoresis-inductively coupled plasma-mass spectrometry hyphenation for the determination at the nanogram scale of metal affinities and binding constants of phosphorylated ligands.

Date publication

mars 2012

Auteurs

Membres identifiés du Cancéropôle Est :
Dr WAGNER Alain

Tous les auteurs :
Varenne F, Bourdillon M, Meyer M, Lin Y, Brellier M, Baati R, Charbonniere LJ, Wagner A, Doris E, Taran F, Hagege A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/22333681

Résumé

A screening strategy based on hyphenated capillary electrophoresis and inductively coupled plasma mass spectrometry (CE-ICP-MS) was developed to classify phosphorylated ligands according to their europium(III) binding affinity in a hydro-organic medium (sodium formate, pH 3.7, H(2)O/MeOH 90:10, v/v). Taking advantage of the high sensibility of ICP-MS for detecting phosphorus, this method enabled to assess the affinity of a variety of phosphorylated compounds, including phosphine oxides, thiophosphines, phosphonates, and phosphinates, in less than 1h and using less than 5 ng of substance. By varying the total europium concentration, complexation constants could be determined according to a sequential multiple run strategy, which proved to be in excellent agreement with the values obtained by UV-Vis absorption spectrophotometric titrations.

Référence

J Chromatogr A. 2012 Mar 16;1229:280-7