Trimethoxyarene as a Highly Ionizable Tag for Reaction Analysis by Atmospheric Pressure Photoionization Mass Spectrometry (APPI/MS): Exploration of Heterocyclic Synthesis.

Date publication

janvier 2012

Auteurs

Membres identifiés du Cancéropôle Est :
Mme SCHAEFFER-REISS Christine , Dr VAN DORSSELAER Alain , Dr WAGNER Alain

Tous les auteurs :
Mathieu BTT, Catala C, Colas C, Schaeffer C, Van Dorsselaer A, Mann A, Wagner A

Résumé

A mass spectrometry (MS) method was developed to rapidly analyze crude reaction mixtures. This method relies on highly effective ionization by atmospheric pressure photoionization (APPI) of molecules with a prosthetic trimethoxyarene (TMOA) residue. In a crude reaction mixture, products resulting from the reaction of the TMOA-labeled substrate will be selectively ionized to afford an easily readable mass spectrum. Interestingly, we noticed that TMOA-labeled molecules were not fragmented and gave the preferred [M + H](+) ion peak. This APPI-MS reaction mixture analysis method was used for the optimization of heterocycle synthesis. By comparing results obtained by APPI/MS, GC, and HPLC analysis, it appeared that a semi-quantification could be achieved by integrating the MS peak intensities.

Référence

. 2012 Jan;1:85-92.