Fiche publication
Date publication
septembre 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NARCE Michel
Tous les auteurs :
Medjdoub A, Merzouk SA, Merzouk H, Chiali FZ, Narce M
Résumé
Pesticides have been shown to possess marked immunotropic activity. The aim of this work was to study the in vitro effects of different concentrations (1-100 mu M) of Mancozeb (fungicide) and Metribuzin (herbicide), on the proliferative responses of human and rat spleen lymphocytes stimulated by concanavalin A (ConA, mitogen), the Th1- (IL-2, INF gamma) and Th2- (IL-4) cytokine secretion and on the intracellular oxidative status. The results showed that Mancozeb significantly reduced ConA lymphocyte proliferation in a dose-dependent manner in both humans and rats. It also decreased IL-2, INF gamma and IL-4 secretion with a a shift away to Th1 phenotype. Metribuzin at low concentrations (1-10 mu M) resulted in activation of ConA stimulated lymphocyte proliferation and cytokine production in both human and rat spleen cells. However, at high concentrations (25-100 mu M), Metribuzin induced a dose-dependent inhibition of lymphocyte proliferation and cytokines. Changes in intracellular levels of reduced Glutathione, hydroperoxides and carbonyl proteins and in the activities of catalase and SOD were observed after Mancozeb and Metribuzin exposure reflecting oxidative stress and DNA damage specially at high concentrations. In conclusion, Mancozeb and Metribuzin had significant immunomodulatory properties with oxidative stress induction at high concentrations. (C) 2011 Elsevier Inc. All rights reserved.
Référence
. 2011 Sep;101(1):27-33.
