G-quadruplex RNA structure as a signal for neurite mRNA targeting.

Date publication

juillet 2011

Auteurs

Membres identifiés du Cancéropôle Est :
Pr MANDEL Jean-Louis

Tous les auteurs :
Subramanian M, Rage F, Tabet R, Flatter E, Mandel JL, Moine H

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/21566646

Résumé

Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis-acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best-known dendritic mRNAs contain a guanine (G)-quadruplex consensus in their 3'-untranslated region. Among these mRNAs, we show by using RNA structure probing that a G-quadruplex is present in the mRNAs of two key postsynaptic proteins: PSD-95 and CaMKIIa. The G-quadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor-responsive manner. Thus, G-quadruplex seems to be a common neurite localization signal.

Référence

EMBO Rep. 2011 Jul 1;12(7):697-704