Fiche publication
Date publication
avril 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Dr VOEGEL Jean-Claude
Tous les auteurs :
Bordenave L, Voegel JC, Durand M, Ponsot JF, Dos Santos P, Menu P
Résumé
The aim of this project was to propose to clinicians a biocompatible vascular substitute, in which the inner surface in contact with blood is lined with autologous endothelial cells to provide non thrombogenicity. The originality of this project lies on the convergence of three main approaches that will be associated: human stem cells differentiated into mature endothelial cells, polyelectrolyte multilayer films including growth factors that could allow cellular adhesion and differentiation, and natural scaffolds (from human umbilical arteries and saphenous veins). This project, essentially multidisciplinary, has allowed to estimate the ability of human and animal EPCs (endothelial progenitor cells) to differentiate into an endothelial lineage, in the presence or not of mechanical stimulation on substrates functionalized with polyelectrolyte multilayer films (PEMFs). to determine the optimal conditions to promote stem cell differentiation and thus optimize the formation of a functional vascular substitute. However. this project has not yet shown on cells the advantages (in terms of saving time, growth factors impact ...) of new generations of polyelectrolyte multilayer films, in or on which growth factors had been adsorbed. The implantation of human re-endothelialized umbilical arteries and saphenous veins as scaffolds have provided very interesting results in rabbits, but disappointing ones in ovins, especially for umbilical arteries. (C) 2011 Elsevier Masson SAS. All rights reserved.
Référence
. 2011 Apr;32(2):142-4.
