FTIR spectroscopic discrimination of Saccharomyces cerevisiae and Saccharomyces bayanus strains.

Date publication

septembre 2010

Auteurs

Membres identifiés du Cancéropôle Est :
Pr SOCKALINGUM Ganesh

Tous les auteurs :
Adt I, Kohler A, Gognies S, Budin J, Sandt C, Belarbi A, Manfait M, Sockalingum GD

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/20921989

Résumé

In this study, we tested the potential of Fourier-transform infrared absorption spectroscopy to screen, on the one hand, Saccharomyces cerevisiae and non-S. cerevisiae strains and, on the other hand, to discriminate between S. cerevisiae and Saccharomyces bayanus strains. Principal components analysis (PCA), used to compare 20 S. cerevisiae and 21 non-Saccharomyces strains, showed only 2 misclassifications. The PCA model was then used to classify spectra from 14 Samos strains. All 14 Samos strains clustered together with the S. cerevisiae group. This result was confirmed by a routinely used electrophoretic pattern obtained by pulsed-field gel electrophoresis. The method was then tested to compare S. cerevisiae and S. bayanus strains. Our results indicate that identification at the strain level is possible. This first result shows that yeast classification and S. bayanus identification can be feasible in a single measurement.

Référence

Can J Microbiol. 2010 Sep;56(9):793-801.