Fluorescent protein recruitment assay for demonstration and analysis of in vivo protein interactions in plant cells and its application to Tobacco mosaic virus movement protein.

Date publication

avril 2010

Auteurs

Membres identifiés du Cancéropôle Est :
Pr MELY Yves , Dr HEINLEIN Manfred , Pr DIDIER Pascal

Tous les auteurs :
Boutant E, Didier P, Niehl A, Mely Y, Ritzenthaler C, Heinlein M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/20070568

Résumé

P>We describe a simple fluorescent protein-based method to investigate interactions with a viral movement protein in living cells that relies on the in vivo re-localization of proteins in the presence of their interaction partners. We apply this method in combination with fluorescence lifetime imaging microscopy (FLIM) to demonstrate that a domain of the Tobacco mosaic virus (TMV) movement protein (MP) previously predicted to mediate protein:protein interactions is dispensable for these contacts. We suggest that this method can be generalized for analysis of other protein interactions in planta.

Référence

Plant J. 2010 Apr 1;62(1):171-7