Conformational switching of the pyridine-pyrimidine-pyridine scaffold for ion-controlled FRET
Fiche publication
Date publication
février 2007
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LEHN Jean-Marie
Tous les auteurs :
Petitjean A, Lehn JM
Lien Pubmed
Résumé
sFluorescence resonance energy transfer (FRET) has become a major tool for the static and dynamic study of conformational changes in biological systems. We report herein the investigation of a switchable pyridine-pyrimidine-pyridine scaffold as a support to ioncontrolled intramolecular FRET. A dissymmetrical switch bearing naphthalene and acridine fluorophores was synthesized and its photo-physical behavior studied. In the neutral state where the molecule adopts a U-shape, the emission of the naphthalene is quenched while a strong emission from the acridine fluorophore is observed, consistent with energy transfer between the naphthalene and the acridine units. The emission of the acridine is also enhanced by the pyridine-induced sensitization (excitation at 280 nm). After introduction of a copper(I) cation which switches the conformation to a W-shape, the complex formed shows the emission of both the naphthalene and acridine units when excited at 280 nm, although coordination also leads to a strong quenching of emission. (c) 2006 Elsevier B.V. All rights reserved.
Référence
Inorg Chim Acta. 2007 Feb 15;360(3):849-56.