Gene expression profiling during hibernation in the European hamster.
Fiche publication
Date publication
septembre 2018
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIOCCA Dominique
Tous les auteurs :
Gautier C, Bothorel B, Ciocca D, Valour D, Gaudeau A, Dupré C, Lizzo G, Brasseur C, Riest-Fery I, Stephan JP, Nosjean O, Boutin JA, Guénin SP, Simonneaux V
Lien Pubmed
Résumé
Hibernation is an exceptional physiological response to a hostile environment, characterized by a seasonal period of torpor cycles involving dramatic reductions of body temperature and metabolism, and arousal back to normothermia. As the mechanisms regulating hibernation are still poorly understood, here we analysed the expression of genes involved in energy homeostasis, torpor regulation, and daily or seasonal timing using digital droplet PCR in various central and peripheral tissues sampled at different stages of torpor/arousal cycles in the European hamster. During torpor, the hypothalamus exhibited strongly down-regulated gene expression, suggesting that hypothalamic functions were reduced during this period of low metabolic activity. During both torpor and arousal, many structures (notably the brown adipose tissue) exhibited altered expression of deiodinases, potentially leading to reduced tissular triiodothyronine availability. During the arousal phase, all analysed tissues showed increased expression of the core clock genes Per1 and Per2. Overall, our data indicated that the hypothalamus and brown adipose tissue were the tissues most affected during the torpor/arousal cycle, and that clock genes may play critical roles in resetting the body's clocks at the beginning of the active period.
Référence
Sci Rep. 2018 Sep 3;8(1):13167