Comprehensive Characterization of the Interaction between Pulsed Electric Fields and Live Cells by Confocal Raman Microspectroscopy.
Fiche publication
Date publication
septembre 2017
Journal
Analytical chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOBINET Cyril, Pr PIOT Olivier
Tous les auteurs :
Azan A, Untereiner V, Descamps L, Merla C, Gobinet C, Breton M, Piot O, Mir LM
Lien Pubmed
Résumé
This study reports a comprehensive analysis of the effect of 100 µs electric pulses on the biochemical composition of live cells using a label-free approach, confocal Raman microspectroscopy. We investigated different regions of interest around the nucleus of the cells and the dose-effect relationship related to different electric pulse parameters. We also extended the study to another cell type. Our results confirmed a previous publication showing that proteins and lipids were highly impacted by the delivery of electric pulses. These chemical changes were similar in different locations around the cell nucleus. By sweeping the field magnitude, the number of electric pulses or their repetition rate, the Raman signatures of live cells appeared to be related to the electropermeabilization state, verified by Yo-Pro-1 uptake. Membrane resealing was monitored by pulsing the cells in reversible or irreversible permeabilization condition at different temperatures. We also demonstrated that the chemical changes in the Raman signatures were cell-dependent even if common features were noticed between the two cell types.
Mots clés
Adipose Tissue, cytology, Animals, Benzoxazoles, chemistry, Cell Line, Electricity, Humans, Mesenchymal Stem Cells, cytology, Mice, Microscopy, Fluorescence, methods, Quinolinium Compounds, chemistry, Spectrum Analysis, Raman, Temperature
Référence
Anal. Chem.. 2017 Sep;: