Low-diluted Phenacetinum disrupted the melanoma cancer cell migration.
Fiche publication
Date publication
juin 2019
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DAUCHEZ Manuel, Pr MARTINY Laurent, Dr TERRYN Christine, Dr SCHNEIDER Christophe, Dr BERQUAND Alexandre
Tous les auteurs :
Fuselier C, Terryn C, Berquand A, Crowet JM, Bonnomet A, Molinari M, Dauchez M, Martiny L, Schneider C
Lien Pubmed
Résumé
Dynamic and reciprocal interactions generated by the communication between tumor cells and their matrix microenvironment, play a major role in the progression of a tumor. Indeed, the adhesion of specific sites to matrix components, associated with the repeated and coordinated formation of membrane protrusions, allow tumor cells to move along a determined pathway. Our study analyzed the mechanism of action of low-diluted Phenacetinum on murine cutaneous melanoma process in a fibronectin matrix environment. We demonstrated a reduction of dispersed cell migration, early and for as long as 24 h, by altering the formation of cell protrusions. Moreover, low-diluted Phenacetinum decreased cell stiffness highly on peripheral areas, due to a disruption of actin filaments located just under the plasma membrane. Finally, it modified the structure of the plasma membrane by accumulating large ordered lipid domains and disrupted B16 cell migration by a likely shift in the balance between ordered and disordered lipid phases. Whereas the correlation between the excess of lipid raft and cytoskeleton disrupting is not as yet established, it is clear that low-diluted Phenacetinum acts on the actin cytoskeleton organization, as confirmed by a decrease of cell stiffness affecting ultimately the establishment of an effective migration process.
Référence
Sci Rep. 2019 Jun 24;9(1):9109