iRGD peptide as effective transporter of CuInZnxS2+x quantum dots into human cancer cells.
Fiche publication
Date publication
octobre 2016
Journal
Colloids and surfaces. B, Biointerfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SCHNEIDER Raphaël
Tous les auteurs :
Przysiecka Ł, Michalska M, Nowaczyk G, Peplińska B, Jesionowski T, Schneider R, Jurga S
Lien Pubmed
Résumé
In this paper, iRGD peptide-mediated quantum dots (QDs) delivery was studied. In the first step, dodecanethiol-capped CuInZnxS2+x (ZCIS) QDs were prepared and subsequently transferred into water using a standard and facile ligand exchange approach involving 3-mercaptopropionic acid (MPA). ZCIS@MPA nanocrystals possess a photoluminescence quantum yield (PL QY) of 25%, a PL emission centered at ca. 640nm and low distributions in size and shape. Next, the iRGD peptide was electrostatically associated to ZCIS@MPA QDs. After cytotoxicity evaluation, the tumor-targeting and penetrating activities of the iRGD/QD assembly were investigated by confocal microscopy. The experiments performed on various cancer cell lines revealed a high penetration ability of the assembly, while the bare QDs were not internalized. Additionally, imaging experiments were conducted on three-dimensional multicellular tumor spheroids in order to mimic the tumor microenvironment in vivo. iRGD/QD assemblies were found to be evenly distributed throughout the whole HeLa spheroid contrary to normal cells where they were not present. Therefore, iRGD/QD assemblies have a great potential to be used as targeted imaging agents and/or nanocarriers specific to cancer cells.
Mots clés
Antineoplastic Agents, chemistry, Bone Neoplasms, drug therapy, Breast Neoplasms, drug therapy, Cell Proliferation, drug effects, Copper, chemistry, Drug Carriers, administration & dosage, Female, HeLa Cells, Humans, Lanthanum, chemistry, Microscopy, Electron, Transmission, Oligopeptides, administration & dosage, Osteosarcoma, drug therapy, Quantum Dots, Spheroids, Cellular, Sulfides, chemistry, Tumor Cells, Cultured, Zinc Compounds, chemistry
Référence
Colloids Surf B Biointerfaces. 2016 Oct;146:9-18