DNA DAMAGE BINDING PROTEIN 2 (DDB2) Shapes the DNA Methylation Landscape.
Fiche publication
Date publication
août 2016
Journal
The Plant cell
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BAUMBERGER Nicolas, Dr HEINTZ Dimitri, Mme COGNAT Valérie
Tous les auteurs :
Schalk C, Drevensek S, Kramdi A, Kassam M, Ahmed I, Cognat V, Graindorge S, Bergdoll M, Baumberger N, Heintz D, Bowler C, Genschik P, Barneche F, Colot V, Molinier J
Lien Pubmed
Résumé
In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at the nexus of DNA repair and chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE-BINDING PROTEIN 2 (DDB2) and the DNA methylation machinery in Arabidopsis thaliana. Loss-of-function mutation in DDB2 leads to genome-wide DNA methylation alterations. Genetic and biochemical evidence indicate that at many repeat loci, DDB2 influences de novo DNA methylation by interacting with ARGONAUTE 4 (AGO4) and by controlling the local abundance of 24-nt short interfering RNAs (siRNAs). We also show that DDB2 regulates active DNA demethylation mediated by REPRESSOR OF SILENCING 1 (ROS1) and DEMETER LIKE-3 (DML3). Together, these findings reveal a role for the DNA repair factor DDB2 in shaping the A. thaliana DNA methylation landscape in the absence of applied genotoxic stress.
Référence
Plant Cell. 2016 Aug 16;: