Soluble HLA-G molecules are increased in lymphoproliferative disorders.
Fiche publication
Date publication
novembre 2003
Journal
Human immunology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DRENOU Bernard
Tous les auteurs :
Sebti Y, Le Friec G, Pangault C, Gros F, Drénou B, Guilloux V, Bernard M, Lamy T, Fauchet R, Amiot L
Lien Pubmed
Résumé
The immunomodulatory properties of soluble human leukocyte antigen G (sHLA-G) explain its potential interest in malignancies. HLA-G frequently transcribed in lymphoproliferative disorders is rarely expressed at cell surface. In this article, we will demonstrate that the plasmatic level of soluble HLA-G was significantly increased in 70% of B chronic lymphocytic leukemia, 53% of non-Hodgkin B lymphoma (B-NHL), and 45% of T-NHL. To explain this variable secretion, the HLA-G secreting cell was searched and was identified as tumoral T4 lymphocytes only in one patient with Sezary syndrome. To approach the mechanisms involved in sHLA-G secretion, the potential role of cytokines has been studied in vitro on T lymphomas. A significant increase of sHLA-G level is observed after activation by cytokines associated with a small increase in the quantity of transcripts using real-time polymerase chain reaction, suggesting an involvement of both transcriptional and post-transcriptional mechanisms. Western Blot analysis reveals no evident variation of the protein expression whatever the conditions, suggesting a continuous secretion and a low intracellular storage. The frequency of the sHLA-G secretion associated to its inhibiting role on T cells and natural killer cells during tumoral lymphoid malignancies suggests a potential role of these molecules as escape mechanism from antitumoral response.
Mots clés
Antibodies, Monoclonal, Blotting, Western, Cytokines, immunology, Cytotoxicity, Immunologic, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, HLA Antigens, blood, HLA-G Antigens, Histocompatibility Antigens Class I, blood, Humans, Killer Cells, Natural, immunology, Leukemia, Lymphocytic, Chronic, B-Cell, immunology, Lymphocyte Activation, Lymphoma, B-Cell, immunology, Lymphoma, T-Cell, immunology, RNA, Messenger, metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sezary Syndrome, immunology, Solubility, T-Lymphocytes, immunology, Transcription, Genetic, Tumor Escape
Référence
Hum. Immunol.. 2003 Nov;64(11):1093-101