Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
Fiche publication
Date publication
juillet 2016
Journal
Biofabrication
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Leite ÁJ, Sarker B, Zehnder T, Silva R, Mano JF, Boccaccini AR
Lien Pubmed
Résumé
Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite. The material could induce the deposition of an apatite layer upon immersion in a biological-like environment to sustain cell attachment and proliferation. Composites were formulated with different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v). Strontium doped BGNPs were also used. EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid. Moreover, the composite constructs could incorporate with high efficiency ibuprofen as a drug model. Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material. Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate. Overall, the composite material showed potential for future applications in bone tissue engineering.
Mots clés
Alginates, chemistry, Animals, Anti-Inflammatory Agents, Non-Steroidal, chemistry, Biocompatible Materials, Bioprinting, Cell Line, Computer-Aided Design, Drug Liberation, Gelatin, chemistry, Glass, chemistry, Hydrogels, chemistry, Ibuprofen, chemistry, Nanoparticles, chemistry, Osteoblasts
Référence
Biofabrication. 2016 07 19;8(3):035005