[Quality control of defrosted cord blood units: results from an inter-laboratory study].
Fiche publication
Date publication
avril 2010
Journal
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DECOT Véronique, Dr POUTHIER Fabienne
Tous les auteurs :
Panterne B, Richard MJ, Sabatini C, Pouthier F, Mouillot L, Bardey D, Boulanger F, Créa S, Dal Cortivo L, Decot V, Fleury-Cappellesso S, Giraud C, Lapierre V, Léauté AG, Le Berre C, Lemarié C, Piard N, Rapatel C, Rosenzwajg M
Lien Pubmed
Résumé
Today, haematopoietic stem cell graft from placental blood concerns more than 15 % of allogeneic grafts. An inter-laboratory study of the quality control of defrosted cord blood units has been coordinated by the French society for cell and tissue bioengineering (SFBCT), with the cord blood bank of Bourgogne Franche-Comté and controlled by the French health products safety agency (Afssaps). The aim of this study is to ensure the inter-laboratory reproducibility of the quality controls practised by the banks during defrosting. The cellular outputs were analyzed according to the defrosting techniques, according to the method used in flow cytometry: single-platform (SP) versus double-platform (DP), or the product nature, i.e. in total blood or miniaturized.
Mots clés
Antigens, CD34, analysis, Blood Cell Count, Blood Preservation, methods, Cell Nucleus, ultrastructure, Clone Cells, cytology, Colony-Forming Units Assay, Cord Blood Stem Cell Transplantation, standards, Cryopreservation, standards, Female, Fetal Blood, France, Hematopoietic Stem Cells, ultrastructure, Humans, Infant, Newborn, Laboratories, Placenta, Pregnancy, Quality Control, Societies, Medical, standards
Référence
Transfus Clin Biol. 2010 Apr;17(2):41-6