Controllable stress patterns over multi-generation timescale in microfluidic devices.
Fiche publication
Date publication
janvier 2018
Journal
Methods in cell biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHARVIN Gilles
Tous les auteurs :
Goulev Y, Matifas A, Charvin G
Lien Pubmed
Résumé
The generation of complex temporal stress patterns may be instrumental to investigate the adaptive properties of individual cells submitted to environmental stress on physiological timescale. However, it is difficult to accurately control stress concentration over time in bulk experiments. Here, we describe a microfluidics-based protocol to induce tightly controllable HO stress in budding yeast while constantly monitoring cell growth with single cell resolution over multi-generation timescale. Moreover, we describe a simple methodology to produce ramping HO stress to investigate the homeostatic properties of the HO scavenging system.
Mots clés
Adaptation, Cell growth, Live-cell imaging, Microfluidics, Stress response
Référence
Methods Cell Biol.. 2018 ;147:29-40