Biohydrogen production in a continuous liquid/gas hollow fiber membrane bioreactor: Efficient retention of hydrogen producing bacteria via granule and biofilm formation.
Fiche publication
Date publication
octobre 2020
Journal
Bioresource technology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ERNST Barbara
Tous les auteurs :
Renaudie M, Dumas C, Vuilleumier S, Ernst B
Lien Pubmed
Résumé
The aim of this work was to develop a continuous liquid/gas membrane bioreactor (L/G MBR), i.e. a fermenting module including hollow fibers membrane for L/G separation, for biohydrogen production by dark fermentation. Originally seeded with sludge from a wastewater treatment plant, the L/G MBR underwent a complete stop for eight months. It was then operated without further reseeding. In the present experiment, performed 551 days after the last reseeding, average hydrogen yield of 1.1 ± 0.2 mol per mol glucose added and hydrogen productivity of 135 ± 22 mL/L/h were reached, with acetate and butyrate as the main metabolite products. DNA sequence analysis revealed that Clostridium beijerinckii, Clostridium pasteurianum and Enterobacter sp. were dominant in liquid outlet, in a biofilm on the surface of the hollow fibers and in microbial granules. The L/G MBR has potential for the concentration and the long-term maintenance of an active hydrogen-producing bacterial community without need for reseeding.
Mots clés
Hollow fibers, Hydrogen, Hydrogen producing bacteria retention, In situ gas extraction, Membrane bioreactor
Référence
Bioresour Technol. 2020 Oct 2;319:124203