Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells.
Fiche publication
Date publication
octobre 2017
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MENARD-MOYON Cécilia, Dr BIANCO Alberto
Tous les auteurs :
Orecchioni M, Bedognetti D, Newman L, Fuoco C, Spada F, Hendrickx W, Marincola FM, Sgarrella F, Rodrigues AF, Ménard-Moyon C, Cesareni G, Kostarelos K, Bianco A, Delogu LG
Lien Pubmed
Résumé
Understanding the biomolecular interactions between graphene and human immune cells is a prerequisite for its utilization as a diagnostic or therapeutic tool. To characterize the complex interactions between graphene and immune cells, we propose an integrative analytical pipeline encompassing the evaluation of molecular and cellular parameters. Herein, we use single-cell mass cytometry to dissect the effects of graphene oxide (GO) and GO functionalized with amino groups (GONH) on 15 immune cell populations, interrogating 30 markers at the single-cell level. Next, the integration of single-cell mass cytometry with genome-wide transcriptome analysis shows that the amine groups reduce the perturbations caused by GO on cell metabolism and increase biocompatibility. Moreover, GONH polarizes T-cell and monocyte activation toward a T helper-1/M1 immune response. This study describes an innovative approach for the analysis of the effects of nanomaterials on distinct immune cells, laying the foundation for the incorporation of single-cell mass cytometry on the experimental pipeline.
Mots clés
Adult, Female, Flow Cytometry, Gene Expression Profiling, Graphite, pharmacology, Humans, Male, Middle Aged, Oxides, pharmacology, Single-Cell Analysis, T-Lymphocytes, cytology, Th1 Cells, immunology
Référence
Nat Commun. 2017 10 24;8(1):1109