Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses.

Fiche publication


Date publication

octobre 2021

Journal

Archives of virology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr PRETET Jean-Luc


Tous les auteurs :
Debernardi A, Jarassier W, Soret C, Mougin C, Alizon S, Bravo IG, Guenat D, Prétet JL, Lepiller Q

Résumé

Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.

Référence

Arch Virol. 2021 Oct 7;: