Human protein-based porous scaffolds as platforms for xeno-free 3D cell culture.
Fiche publication
Date publication
février 2022
Journal
Advanced healthcare materials
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Santos SC, Custódio CA, Mano JF
Lien Pubmed
Résumé
Extracellular matrix and protein-based biomaterials emerged as attractive sources to produce scaffolds due to their great properties regarding biocompatibility and bioactivity. In addition, there are concerns regarding the use of animal-derived supplements in cell culture not only due to risk of transmission of xenogeneic contaminants and antigens but also due to ethical issues associated with collection methods. Herein, we propose novel human protein-derived porous scaffolds produced from platelet lysates (PL) as platforms for xeno-free three-dimensional (3D) cell culture. Human PL were chemically modified with methacryloyl groups (PLMA) to make them photocrosslinkable and used as precursor material to produce PLMA-based sponges. The herein reported human-based sponges have highly tunable morphology and mechanical properties, with an internal porous structure and Young's modulus dependent on the concentration of the polymer. Human adipose-derived stem cells (hASCs) were cultured on top of PLMA sponges to validate their use for 3D cell culture in xeno-free conditions. After 14 days hASCs remained viable, and results show that cells were able to proliferate during time even in the absence of animal-derived supplementation. Our study reveals for the first time that such scaffolds can be promising platforms for culture of human cells avoiding the use of any animal-derived supplement. This article is protected by copyright. All rights reserved.
Mots clés
Human platelet lysates, personalized medicine, sponges, tissue engineering, xeno-free cell culture
Référence
Adv Healthc Mater. 2022 Feb 19;:e2102383