Multimodal image reconstruction from tomographic diffraction microscopy data.
Fiche publication
Date publication
juillet 2022
Journal
Journal of microscopy
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAEBERLE Olivier
Tous les auteurs :
Abbessi R, Verrier N, Taddese AM, Laroche S, Debailleul M, Lo M, Courbot JB, Haeberlé O
Lien Pubmed
Résumé
Tomographic Diffraction Microscopy (TDM) is a tool of choice for high-resolution, marker-less 3D imaging of biological samples. Based on a generalization of Digital Holographic Microscopy (DHM) with full control of the sample's illumination, TDM measures, from many illumination directions, the diffracted fields in both phase and amplitude. Photon budget associated to TDM imaging is low. Therefore, TDM is not limited by photo-toxicity issues. The recorded information makes it possible to reconstruct 3D refractive index distribution (with both refraction and absorption contributions) of the object under scrutiny, without any staining. In this contribution, we show an alternate use of this information. A tutorial for multimodal image reconstruction is proposed. Both intensity contrasts and phase contrasts are proposed, from the image formation model to the final reconstruction with both 2D and 3D rendering, turning TDM into a kind of "universal" digital microscope. This article is protected by copyright. All rights reserved.
Mots clés
holography, microscopy, tomography
Référence
J Microsc. 2022 07 1;: