A lipid transfer protein ensures nematode cuticular impermeability.
Fiche publication
Date publication
novembre 2022
Journal
iScience
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BIRCK Catherine, Dr POTERSZMAN Arnaud
Tous les auteurs :
Njume FN, Razzauti A, Soler M, Perschin V, Fazeli G, Bourez A, Delporte C, Ghogomu SM, Poelvoorde P, Pichard S, Birck C, Poterszman A, Souopgui J, Van Antwerpen P, Stigloher C, Vanhamme L, Laurent P
Lien Pubmed
Résumé
The cuticle of is impermeable to chemicals, toxins, and pathogens. However, increased permeability is a desirable phenotype because it facilitates chemical uptake. Surface lipids contribute to the permeability barrier. Here, we identify the lipid transfer protein GMAP-1 as a critical element setting the permeability of the cuticle. A deletion mutant increases cuticular permeability to sodium azide, levamisole, Hoechst, and DiI. Expressing GMAP-1 in the hypodermis or transiently in the adults is sufficient to rescue this permeability phenotype. GMAP-1 protein is secreted from the hypodermis to the aqueous fluid filling the space between collagen fibers of the cuticle. , GMAP-1 protein binds phosphatidylserine and phosphatidylcholine while , GMAP-1 sets the surface lipid composition and organization Altogether, our results suggest GMAP-1 secreted by hypodermis shuttles lipids to the surface to form the permeability barrier of .
Mots clés
Biological sciences, molecular biology, physiology
Référence
iScience. 2022 11 18;25(11):105357