Infrared nanospectroscopic imaging of DNA molecules on mica surface.
Fiche publication
Date publication
novembre 2022
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr PIETREMENT Olivier
Tous les auteurs :
Custovic I, Pocholle N, Bourillot E, Lesniewska E, Piétrement O
Lien Pubmed
Résumé
Significant efforts have been done in last two decades to develop nanoscale spectroscopy techniques owning to their great potential for single-molecule structural detection and in addition, to resolve open questions in heterogeneous biological systems, such as protein-DNA complexes. Applying IR-AFM technique has become a powerful leverage for obtaining simultaneous absorption spectra with a nanoscale spatial resolution for studied proteins, however the AFM-IR investigation of DNA molecules on surface, as a benchmark for a nucleoprotein complexes nanocharacterization, has remained elusive. Herein, we demonstrate methodological approach for acquisition of AFM-IR mapping modalities with corresponding absorption spectra based on two different DNA deposition protocols on spermidine and Ni pretreated mica surface. The nanoscale IR absorbance of distinctly formed DNA morphologies on mica are demonstrated through series of AFM-IR absorption maps with corresponding IR spectrum. Our results thus demonstrate the sensitivity of AFM-IR nanospectroscopy for a nucleic acid research with an open potential to be employed in further investigation of nucleoprotein complexes.
Mots clés
Microscopy, Atomic Force, methods, DNA, Aluminum Silicates, chemistry, Nucleoproteins, Spectrophotometry, Infrared, methods
Référence
Sci Rep. 2022 11 8;12(1):18972