Interaction between Movement Proteins of .
Fiche publication
Date publication
décembre 2022
Journal
Viruses
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEINLEIN Manfred
Tous les auteurs :
Atabekova AK, Lazareva EA, Lezzhov AA, Solovieva AD, Golyshev SA, Skulachev BI, Solovyev ID, Savitsky AP, Heinlein M, Morozov SY, Solovyev AG
Lien Pubmed
Résumé
Movement proteins (MPs) of plant viruses enable the translocation of viral genomes from infected to healthy cells through plasmodesmata (PD). The MPs functions involve the increase of the PD permeability and routing of viral genome both to the PD entrance and through the modified PD. encodes two MPs, termed BMB1 and BMB2, which act in concert to accomplish virus cell-to-cell transport. BMB1, representing an NTPase/helicase domain-containing RNA-binding protein, localizes to the cytoplasm and the nucleoplasm. BMB2 is a small hydrophobic protein that interacts with the endoplasmic reticulum (ER) membranes and induces local constrictions of the ER tubules. In plant cells, BMB2 localizes to PD-associated membrane bodies (PAMBs) consisting of modified ER tubules and directs BMB1 to PAMBs. Here, we demonstrate that BMB1 and BMB2 interact in vitro and in vivo, and that their specific interaction is essential for BMB2-directed targeting of BMB1 to PAMBs. Using mutagenesis, we show that the interaction involves the C-terminal BMB1 region and the N-terminal region of BMB2.
Mots clés
Hibiscus green spot virus, cell-to-cell movement, higrevirus, movement protein, plant virus, plasmodesmata, protein-protein interaction, virus transport
Référence
Viruses. 2022 12 8;14(12):