Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with cells.
Fiche publication
Date publication
décembre 2023
Journal
Beilstein journal of nanotechnology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEURTAULT Béatrice, Dr RABINEAU Morgane
Tous les auteurs :
Equy E, Hirtzel J, Hellé S, Heurtault B, Mathieu E, Rabineau M, Ball V, Ploux L
Lien Pubmed
Résumé
Inspired by the eumelanin aggregates in human skin, polydopamine nanoparticles (PDA NPs) are promising nanovectors for biomedical applications, especially because of their biocompatibility. We synthesized and characterized fluorescent PDA NPs of 10-25 nm diameter based on a protein containing a lysine-glutamate diad (bovine serum albumin, BSA) and determined whether they can penetrate and accumulate in bacterial cells to serve as a marker or drug nanocarrier. Three fluorescent PDA NPs were designed to allow for tracking in three different wavelength ranges by oxidizing BSA/PDA NPs (Ox-BSA/PDA NPs) or labelling with fluorescein 5-isothiocyanate (FITC-BSA/PDA NPs) or rhodamine B isothiocyanate (RhBITC-BSA/PDA NPs). FITC-BSA/PDA NPs and RhBITC-BSA/PDA NPs penetrated and accumulated in both cell wall and inner compartments of () cells. The fluorescence signals were diffuse or displayed aggregate-like patterns with both labelled NPs and free dyes. RhBITC-BSA/PDA NPs led to the most intense fluorescence in cells. Penetration and accumulation of NPs was not accompanied by a bactericidal or inhibitory effect of growth as demonstrated with the Gram-negative species and confirmed with a Gram-positive bacterial species (). Altogether, these results allow us to envisage the use of labelled BSA/PDA NPs to track bacteria and carry drugs in the core of bacterial cells.
Mots clés
Escherichia coli, accumulation, albumin, antibacterial, fluorescence, nanoparticles, penetration, polydopamine
Référence
Beilstein J Nanotechnol. 2023 12 22;14:1208-1224