Cryo-EM structure of the inactive ribosome complex accumulated in chick embryo cells in cold-stress conditions.
Fiche publication
Date publication
février 2024
Journal
FEBS letters
Auteurs
Membres identifiés du Cancéropôle Est :
Dr YUSUPOV Marat
Tous les auteurs :
Nurullina L, Terrosu S, Myasnikov AG, Jenner LB, Yusupov M
Lien Pubmed
Résumé
Here, we present the high-resolution structure of the Gallus gallus 80S ribosome obtained from cold-treated chicken embryos. The translationally inactive ribosome complex contains elongation factor eEF2 with GDP, SERPINE1 mRNA binding protein 1 (SERBP1) and deacylated tRNA in the P/E position, showing common features with complexes already described in mammals. Modeling of most expansion segments of G. gallus 28S ribosomal RNA allowed us to identify specific features in their structural organization and to describe areas where a marked difference between mammalian and avian ribosomes could shed light on the evolution of the expansion segments. This study provides the first structure of an avian ribosome, establishing a model for future structural and functional studies on the translational machinery in Aves.
Mots clés
Gallus gallus, SERBP1, avian ribosome structure, cold exposure
Référence
FEBS Lett. 2024 02 23;: