A Kinetic Approach to Synergize Bactericidal Efficacy and Biocompatibility in Silver-Based Sol-Gel Coatings.
Fiche publication
Date publication
juin 2024
Journal
ACS omega
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHERIOUX Frédéric
Tous les auteurs :
Zwingelstein T, Figarol A, Luzet V, Crenna M, Bulliard X, Finelli A, Gay J, Lefèvre X, Pugin R, Laithier JF, Chérioux F, Humblot V
Lien Pubmed
Résumé
Silver ions are antimicrobial agents with powerful action against bacteria. Applications in surface treatments, as Ag-functionalized sol-gel coatings, are expected in the biomedical field to prevent contaminations and infections. The potential cytotoxicity of Ag cations toward human cells is well known though. However, few studies consider both the bactericidal activity and the biocompatibility of the Ag-functionalized sol-gels. Here, we demonstrate that the cytotoxicity of Ag cations is circumvented, thanks to the ability of Ag cations to kill () much faster than normal human dermal fibroblasts (NHDFs). This phenomenon was investigated in the case of two silver nitrate-loaded sol-gel coatings: one with 0.5 w/w% Ag cations and the second with 2.5 w/w%. The maximal amount of released Ag ions over time (0.25 mg/L) was ten times lower than the minimal inhibition (MIC) and minimal bactericidal (MBC) concentrations (respectively, 2.5 and 16 mg/L) for and twice lower to the minimal cytotoxic concentration (0.5 mg/L) observed in NHDFs. were killed 8-18 times, respectively, faster than NHDFs by silver-loaded sol-gel coatings. This original approach, based on the kinetic control of the biological activity of Ag cations instead of a concentration effect, ensures the bactericidal protection while maintaining the biocompatibility of the Ag cation-functionalized sol-gels. This opens promising applications of silver-loaded sol-gel coatings for biomedical tools in short-term or indirect contacts with the skin.
Référence
ACS Omega. 2024 06 11;9(23):24574-24583