Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation.
Fiche publication
Date publication
août 2012
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SOCKALINGUM Ganesh
Tous les auteurs :
Ait-Belkacem D, Guilbert M, Roche M, Duboisset J, Ferrand P, Sockalingum G, Jeannesson P, Brasselet S
Lien Pubmed
Résumé
Polarization resolved second harmonic generation (PSHG) is developed to study, at the microscopic scale, the impact of aging on the structure of type I collagen fibrils in two-dimensional coatings. A ribose-glycated collagen is also used to mimic tissue glycation usually described as an indicator of aging. PSHG images are analyzed using a generic approach of the molecular disorder information in collagen fibrils, revealing significant changes upon aging, with a direct correlation between molecular disorder and fibril diameters.
Référence
J Biomed Opt. 2012 Aug;17(8):080506-1