Expression of chondrogenic genes by undifferentiated vs. differentiated human mesenchymal stem cells using array technology.
Fiche publication
Date publication
janvier 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BENSOUSSAN Danièle, Pr GILLET Pierre, Pr MAINARD Didier, Dr ROEDER Emilie
Tous les auteurs :
Henrionnet C, Roeder E, Gillet R, Galois L, Bensoussan D, Mainard D, Netter P, Gillet P, Pinzano A
Lien Pubmed
Résumé
This study investigated the gene expression profile of human mesenchymal stem cells seeded in collagen sponge for 28 days in three different mediums: (1) basal medium as control containing ITS alone, (2) ITS+TGF-beta1 alone or (3) ITS 1% supplemented sequentially by TGF-beta1 (D3-D14) followed by BMP-2 (D15-D28). Differential expression of 84 genes implicated in chondrogenic and osteogenic differentiation of MSCs was analyzed at D28 by real-time RT-PCR array technology. TGF-beta1 alone down-regulated two genes, CD36 and cathepsin K. Sixteen genes were significantly up-regulated, notably type 2 and type 10 collagens, COMP and Sox9. The sequential combination of TGF-beta1 and BMP-2 produced a similar profile with prominent expression of type 2 collagen and the alkaline phosphatase gene. Interestingly, in this in vitro condition, RUNX2 was not up-regulated, suggesting that the sequential combination of TGF-beta1/BMP2 enhances the hypertrophic chondrogenic profile without turning towards the osteoblastic pathway.
Référence
Biomed Mater Eng. 2010 Jan 1;20(3):175-81.