CATALYTIC PROPERTIES OF THE EUKARYOTIC EXOSOME.
Fiche publication
Date publication
janvier 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SERAPHIN Bertrand
Tous les auteurs :
Chlebowski A, Tomecki R, Lopez MEG, Seraphin B, Dziembowski A
Lien Pubmed
Résumé
The eukaryotic exosome complex is built around the backbone of a 9-subunit ring similar to phosporolytic ribonucleases such as RNase PH and polynucleotide phosphorylase (PNPase). Unlike those enzymes, the ring is devoid of any detectable catalytic activities, with the possible exception of the plant version of the complex. Instead, the essential RNA decay capability is supplied by associated hydrolytic ribonucleases belonging to the Dis3 and Rrp6 families. Dis3 proteins are endowed with two different activities: the long known processive 3'-5' exonucleolytic one and the recently discovered endonucleolytic one. Rrp6 proteins are distributive exonucleases. This chapter will review the current knowledge about the catalytic properties of theses nucleases and their interplay within the exosome holocomplex.
Référence
Adv Exp Med Biol. 2010;702:63-78.