Saturated structured confocal microscopy with theoretically unlimited resolution
Fiche publication
Date publication
septembre 2009
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAEBERLE Olivier
Tous les auteurs :
Haeberle O, Simon B
Lien Pubmed
Résumé
The resolution of fluorescence microscopes is limited by diffraction, which determines the extension of their point spread functions. We propose and study numerically a simple method, based on a combination of subtraction microscopy with regular and annular excitation beams, which permits to double the resolution compared to wide field microscopy. When combined with the fluorescence saturation phenomenon, this approach would be able to deliver a resolution of a few tens of nanometers. (c) 2009 Elsevier B.V. All rights reserved.
Référence
Opt Commun. 2009 Sep 15;282(18):3657-64