Preparation of flat gold terraces for protein chip developments
Fiche publication
Date publication
juin 2009
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid, Dr ELIE-CAILLE Céline
Tous les auteurs :
Elie-Caille C, Rauch JY, Rouleau A, Boireau W
Lien Pubmed
Résumé
A simple method to prepare. at gold terraces on mica for atomic force microscopy biomolecular characterisation is described. The procedure includes preheating of the substrate, metal deposition and an annealing step. All of these steps are at elevated temperatures (300-420 degrees C). This approach allows one to prepare large. at gold terraces (200-500 nm), which constitute ideal substrates for visualisation and characterisation of a self-assembly monolayer of biomolecules at the nanoscale. The authors illustrated this potential of characterisation with the reconstitution of a protein monolayer.
Référence
Micro Nano Lett. 2009 Jun;4(2):88-94