Integrated love wave device dedicated to biomolecular interactions measurements in aqueous media

Fiche publication


Date publication

septembre 2007

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid


Tous les auteurs :
Blondeau-Patissier V, Boireau W, Cavallier B, Lengaigne G, Daniau W, Martin G, Ballandras S

Résumé

Mass-sensitive electro-acoustic devices such as surface acoustic wave (SAW) micro-balances, capable to operate with aqueous media are particularly favorable for the development of biosensors. Their dimensions and physical properties offer a large potential in biological fluid investigations, especially for measuring physical phenomenon ( mass deposition, adsorption, pressure.). In this work, we propose a specific grating configuration to lower the influence of viscosity of fluids which reduces the signal dynamics of the surface wave transducers. A dedicated liquid cell also has been developed to isolate the electro-active part of the device. The fabrication of the cell is achieved using the SU-8(TM) photo-resist, allowing for manufacturing thick structures preventing any contact between the tested liquids and the transducers. Furthermore, the sensing area has been optimized to optimize the sensor gravimetric sensitivity. The operation of the sensor is illustrated by detecting bovine serum albumin (BSA) adsorption in the sensing area.

Référence

Sensors. 2007 Sep;7(9):1992-2003