Evaluation by Q-RTPCR of the efficacy of ribavirin complexed with beta-cyclodextrin against measles virus in a mouse encephalitis model.
Fiche publication
Date publication
décembre 2006
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JEULIN Hélène
Tous les auteurs :
Jeulin H, Grancher N, Kedzierewicz F, Le Faou AE, Venard V
Lien Pubmed
Résumé
The objective of this work was to study the antiviral activity of ribavirin on measles encephalitis infection when using cyclodextrins as carriers. The use of cyclic oligosaccharides can promote the activity of many drugs and the benefit of the association of ribavirin with beta-cyclodextrin has already been demonstrated in vitro. Intracranial inoculation of the rodent adapted neurovirulent CAM/RB strain of measles virus induces encephalitis in CBA/ca mice. The antiviral activity of the complex ribavirin/beta-cyclodextrinat molar ratio 1:1 has been evaluated in vivo in the above encephalitis model. CBA/ca mice were; treated with daily intraperitoneal injection of ribavirin (40 mg/kg) with or without cyclodextrin. The viral load in the brain of mice was quantified by real-time Reverse transcription-Polymerase chain reaction. Treatment of mice by the complex ribavirin/beta-cyclodextrin (1:1) by intraperitoneal route decreases the viral load in the brain of 1.1 and 0.7 log(10) Eq copies x mL(-1) compared to distillated water and ribavirin treatment, respectively. At the same time, free ribavirin injection shows a negligible difference compared to treatment by distillated water. (c) 2006 Elsevier Masson SAS. All rights reserved.
Référence
Pathol Biol (Paris). 2006 Dec;54(10):541-4