Low-cost fibered fluorescence spectroscopic probe designed for biomolecular film characterization.
Fiche publication
Date publication
mai 2006
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid, Dr PIERALLI Christian, Dr WACOGNE Bruno
Tous les auteurs :
Prevost S, Pieralli C, Wacogne B, Boireau W
Lien Pubmed
Résumé
We present a new combination of a fluorescent film deposited onto a chip and a new kind of low-cost optical fluorescence probe. The probe consists of an optical fiber which guides the excitation beam to the chip and a second one which collects the fluorescence light toward a spectrometer slotted into a PC. The resulting data are then processed to extract the pertinent information. This system is designed for the detection of DNA but, at present, we have achieved the detection of functionalized lipidic vesicles and studied the photobleaching process of such films.
Référence
Laser Phys. 2006 May;16(5):788-93.