Multi-kernel deconvolution applied to confocal fluorescence microscopy with engineered point spread function.
Fiche publication
Date publication
janvier 2006
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAEBERLE Olivier
Tous les auteurs :
Simon B, Haeberle O
Lien Pubmed
Résumé
Fluorescence microscopy is a powerful technique in biology, because of the immense variety of markers now available. Compared to other methods, its resolution is however limited. In wide-field microscopy, the technique of structured illumination permits to improve the lateral resolution by a factor of two, even surpassing confocal microscopy, which permits a theoretical gain of about 40%. We propose an alternate technique, combining laterally interfering focused beams, which should permit the same gain of resolution in a confocal microscope. Furthermore, this technique, combined with multiple acquisition and multikernel deconvolution, permits a better object reconstruction than classical monokernel deconvolution using a regular excitation point spread function. [DOI: 10.2971/jeos.2006.06028]
Référence
J Eur Opt Soc-rapid Publ. 2006;1:06028.