Bioengineering and characterization of DNA-protein assemblies floating on supported membranes.
Fiche publication
Date publication
janvier 2002
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid
Tous les auteurs :
Boireau W, Bombard S, Sari MA, Pompon D
Lien Pubmed
Résumé
A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors.
Référence
Biotechnol Bioeng. 2002 Jan 20;77(2):225-31.