Sensitivity to taxoid derivatives of a newly established human endometrioid ovarian adenocarcinoma radioresistant cell line.
Fiche publication
Journal
Anticancer research
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MERLIN Jean-Louis
Tous les auteurs :
Griffon G, Merlin JL, Parache RM, Martinet N, Marchal S, Nabet F, Wellman M, Barlier C, Marchal C
Lien Pubmed
Résumé
An endometrioid ovarian adenocarcinoma cell line CAVEOC-2 was characterized. Maintained in monolayered culture, CAVEOC-2 cells exhibited a 33-hr doubling time. When xenografted into nude mice, these cells produced fast growing tumors. Colony-forming efficiency in agar was 50%. DNA index was 1.5 and cytogenetic analysis showed a triploid karyotype. CAVEOC-2 cells did not express mdr-1 gene and were chemosensitive to doxorubicin (IC50 = 1.82 +/- 0.76 mumol/l), paclitaxel (IC50 = 3.33 +/- 0.26 nmol/l) and docetaxel (IC50 = 0.68 +/- 0.28 nmol/l), while they showed an intermediate sensitivity to cisplatin (IC50 = 9.40 +/- 1.02 mumol/l). CAVEOC-2 cells seemed highly radioresistant (SF2 = 0.81, alpha = 0.02 Gy-1, beta = 0.025 Gy2, and MID = 4.31 Gy). Activities of glutathione S transferase and gamma-glutamyl transpeptidase were respectively 23.5- and 3.4- fold higher than those of sensitive A2780 cell line. These characteristics make the CAVEOC-2 cells a suitable model for the study of human endometrioid ovarian adenocarcinoma.
Mots clés
Animals, Antineoplastic Agents, Phytogenic, pharmacology, Base Sequence, Carcinoma, Endometrioid, drug therapy, Cell Division, drug effects, DNA, Neoplasm, analysis, Doxorubicin, pharmacology, Drug Screening Assays, Antitumor, Female, Flow Cytometry, Gene Expression, Glutathione Peroxidase, metabolism, Glutathione Transferase, metabolism, Humans, Immunohistochemistry, Karyotyping, Mice, Mice, Nude, Middle Aged, Molecular Sequence Data, Ovarian Neoplasms, drug therapy, P-Glycoprotein, genetics, Paclitaxel, analogs & derivatives, Ploidies, RNA, Messenger, metabolism, Radiation Tolerance, Taxoids, Tumor Cells, Cultured, gamma-Glutamyltransferase, metabolism
Référence
Anticancer Res.. ;16(1):177-87