Multiple inputs ensure yeast cell size homeostasis during cell cycle progression.
Fiche publication
Date publication
juillet 2018
Journal
eLife
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MOLINA Nacho, Dr CHARVIN Gilles
Tous les auteurs :
Garmendia-Torres C, Tassy O, Matifas A, Molina N, Charvin G
Lien Pubmed
Résumé
Coordination of cell growth with division is essential for proper cell function. In budding yeast, although some molecular mechanisms responsible for cell size control during G1 have been elucidated, the mechanism by which cell size homeostasis is established remains to be discovered. Here, we developed a new technique based on quantification of histone levels to monitor cell cycle progression in individual cells with unprecedented accuracy. Our analysis establishes the existence of a mechanism controlling bud size in G2/M that prevents premature onset of anaphase, and controls the overall size variability. While most G1 mutants do not display impaired size homeostasis, mutants in which cyclin B-Cdk regulation is altered display large size variability. Our study thus demonstrates that size homeostasis is not controlled by a G1-specific mechanism alone but is likely to be an emergent property resulting from the integration of several mechanisms that coordinate cell and bud growth with division.
Mots clés
S. cerevisiae, cell biology, cell cycle, computational biology, microfluidics, size control, systems biology
Référence
Elife. 2018 Jul 4;7: