Orthogonal Operation of Constitutional Dynamic Networks Consisting of DNA-Tweezer Machines.
Fiche publication
Date publication
décembre 2017
Journal
ACS nano
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LEHN Jean-Marie
Tous les auteurs :
Yue L, Wang S, Cecconello A, Lehn JM, Willner I
Lien Pubmed
Résumé
Overexpression or down-regulation of cellular processes are often controlled by dynamic chemical networks. Bioinspired by nature, we introduce constitutional dynamic networks (CDNs) as systems that emulate the principle of the nature processes. The CDNs comprise dynamically interconvertible equilibrated constituents that respond to external triggers by adapting the composition of the dynamic mixture to the energetic stabilization of the constituents. We introduce a nucleic acid-based CDN that includes four interconvertible and mechanically triggered tweezers, AA', BB', AB' and BA', existing in closed, closed, open, and open configurations, respectively. By subjecting the CDN to auxiliary triggers, the guided stabilization of one of the network constituents dictates the dynamic reconfiguration of the structures of the tweezers constituents. The orthogonal and reversible operations of the CDN DNA tweezers are demonstrated, using T-A·T triplex or K-stabilized G-quadruplex as structural motifs that control the stabilities of the constituents. The implications of the study rest on the possible applications of input-guided CDN assemblies for sensing, logic gate operations, and programmed activation of molecular machines.
Mots clés
DNAzyme, G-quadruplex, nanobiotechnology, nucleic acid, strand displacement, supramolecular structure, triplex
Référence
ACS Nano. 2017 Dec 26;11(12):12027-12036