Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine.
Fiche publication
Date publication
octobre 2017
Journal
International journal of molecular sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr BOULAGNON-ROMBI Camille, Dr VELARD Frédéric, Dr KERDJOUDJ Halima
Tous les auteurs :
Aubert L, Dubus M, Rammal H, Bour C, Mongaret C, Boulagnon-Rombi C, Garnotel R, Schneider C, Rahouadj R, Laurent C, Gangloff SC, Velard F, Mauprivez C, Kerdjoudj H
Lien Pubmed
Résumé
Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton's jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications.
Mots clés
Cellular Microenvironment, Collagen, Humans, Mesenchymal Stromal Cells, Preservation, Biological, methods, Regenerative Medicine, instrumentation
Référence
Int J Mol Sci. 2017 Oct 21;18(10):